RevoluGen Ltd

Fire Monkey HMW-DNA Extraction Workflows RevoluGen Ltd LGC SPT Labtech Thermo Fisher Scientific Oxford Nanopore Technologies Illumina

Fully automated Kingfisher Thermo Fisher Scientific Fire Monkey beads HMW-DNA extraction from white blood cells. No size selection post-extraction LSK114/MinION Oxford Nanopore Technologies https://lnkd.in/eTSseG8i

The same DNA extract could be used for short- and hybrid (short/long)-readworkflows, as already shown for samples extracted with Fire Monkey spin column kit providing users with a unique one stop sequencing-agnostic automated platform.

Many thanks to Qura Shah for the Kingfisher Apex Thermo Fisher Scientific demo! We RevoluGen Ltd will be developing magnetic beads extraction protocols for hybrid Illumina Oxford Nanopore Technologies sequencing.

The same DNA extract could be used for short- and hybrid (short/long)-readworkflows, as already shown for samples extracted with Fire Monkey spin column kit providing users with a unique one stop sequencing-agnostic automated platform.

Re-analysis of an outbreak of Shiga toxin-producing Escherichia coli O157:H7 associated with raw drinking milk using Nanopore sequencing UK Health Security Agency DNA extraction, library preparation, Oxford Nanopore Technologies sequencing and data processing High-molecular weight (HMW) genomic DNA was extracted and purified using the Revolugen Fire Monkey HMW DNA extraction kit RevoluGen Ltd. https://lnkd.in/exxFzaYG

Fire Monkey RevoluGen Ltd granted patent HMW-DNA extraction chemistry for hybrid Oxford Nanopore Technologies & Illumina reads, using: Spin columns https://lnkd.in/eduvJhsd Spin filter plates Tecan https://lnkd.in/eEX6Fm4U https://lnkd.in/ebSPq3Ws Positive pressure filter plates Tecan https://lnkd.in/ef89dRqD Manual magnetic beads https://lnkd.in/ev362z_J Automated magnetic beads LGC SPT Labtech https://lnkd.in/e8AFkwye https://lnkd.in/eJiyQ-E5 And very soon, Thermo Fisher Scientific Kingfisher!!

We are excited to announce the results of our collaboration with SPT Labtech to demonstrate automation of RevoluGen’s Fire Monkey™ High Molecular Weight (HMW) DNA extraction chemistry in a bead-based format using SPT Labtech’s firefly® automated liquid handling platform. Representing a meaningful advance in sample preparation efficiency for long-read (and hybrid) DNA sequencing, the automated protocol enables the processing of up to 96 samples in parallel to prepare HMW DNA suitable for library preparation for sequencing in under two hours. Read the announcement here: https://lnkd.in/ecf_va9c See the SPT Labtech Application Note here: Application Note using Fire Monkey and firefly on Oxford Nanopore Technologies: https://lnkd.in/e7EwAmiA #dnasequencing #DNAextraction #HMWDNA #DNA #automation

Terrific piece in Nature about why speed matters when you are using genomics to answer health questions “But perhaps the greatest excitement centres on ‘long-read’ technologies, particularly those developed by UK firm Oxford Nanopore Technologies (ONT). Long reads are valuable for documenting gene duplications, deletions and other structural changes that are challenging to capture with short-read methods. Importantly, this technology can also be used to detect other ‘omics’ indicators when an ultra-rapid response is required, such as for cancer (see ‘A matter of minutes’).” Oxford Nanopore Technologies Emma Stanton Kathleen C. Barnes Gordon Sanghera Tonya McSherry Cerissa French jonathan edgeworth Thomas Bray - 唐睿 Julie Collens Stephanie Abernathy Renner Rosemary Sinclair Dokos Rich Compton Carolyn Tregidgo

"The fire Monkey kit (RevoluGen Ltd, UK) was used to extract high quality High Molecular Weight DNA from E. faecalis ST40. Both isolates were then sequenced on Illumina and Oxford Nanopore Technologies platforms." Quadram Institute FakA impacts antiseptic susceptibility in Staphylococcus aureus and Enterococcus faecalis https://lnkd.in/epdta5-m